产品货号:
GS4407
中文名称:
重组小鼠TNF-α
英文名称:
Recombinant Murine Tumor Necrosis Factor-alpha
产品规格:
5μg
发货周期:
1~3天
产品价格:
询价
General Description:
Tumor necrosis factor alpha (TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.The biologically active native form of TNF-αis reportedly a trimer.Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Storage:
This lyophilized preparation is stable at 2~8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2~8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight:
Approximately 17.4kDa.The recombinant murine TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Purity:
> 98% by SDS-PAGE and HPLC analyses.
Source:
Escherichia coli.
Biological Activity:
Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1ng/mL,corresponding to a specific activity of> 1.0×107 IU/mg in the presence of actinomycin D.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS,pH7.2.
Endotoxin:
Less than 1 EU/μg of rMuTNF-α as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1~1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Tumor necrosis factor alpha (TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.The biologically active native form of TNF-αis reportedly a trimer.Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Storage:
This lyophilized preparation is stable at 2~8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2~8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight:
Approximately 17.4kDa.The recombinant murine TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Purity:
> 98% by SDS-PAGE and HPLC analyses.
Source:
Escherichia coli.
Biological Activity:
Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1ng/mL,corresponding to a specific activity of> 1.0×107 IU/mg in the presence of actinomycin D.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS,pH7.2.
Endotoxin:
Less than 1 EU/μg of rMuTNF-α as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1~1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
相关搜索:重组小鼠TNF-α,TNF-α重组蛋白,Recombinant Murine Tumor Necrosis Factor-alpha